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HSV 1 and 2 by PCR

herpes simplex virus types 1 and 2

Detection and differentiation of Herpes simplex virus types 1 and 2 by realtime PCR.

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Submit only one (1) of the following specimens:
ThinPrep® Pap specimen
APTIMA® Multitest Swab (orange) specimen
APTIMA® Endocervical Swab specimen
Viral transport media swab.

• ThinPrep^® specimens – please refer to instructions in Collection of Pap Specimens Using Thin Prep.^® Collect material as for a routine Pap specimen; it is important that cervical mucus be removed.
• Endocervical swab specimens – please refer to instructions in Collection of Endocercvial Swab Specimens Using the APTIMA^® Unisex Swab Specimen Collection Kit.
• Vaginal or lesion swab – please refer to instructions in Collection of Vaginal Swab Specimens Using the APTIMA^® Multitest Swab Specimen Kit.
• Swab specimen – swab the lesion, using sufficient pressure to ensure good contact of the swab with the lesion. Gently rotate the swab. Remove the cap from the specimen transport tube and immediately place the swab into the tube. Carefully break the shaft against the side of the tube at the scoreline, then discard the top portion of the swab shaft. Tightly recap the tube.

Swab specimens are stable if stored at 2-30°C for 60 days.
However, liquid-based cytology specimens are stable if stored at 2-30°C for 30 days.

• APTIMA^® swab vial specimens submitted without a swab will be rejected.
• Click here for Specimen Rejection Criteria.

2-4 days

Real time PCR

• Test results may be affected by improper/inadequate specimen collection.
• A negative result indicates that HSV-1 and -2 DNA are not present at detectable quantities. It does not indicate that an individual has never been exposed to HSV-1 and/or 2, and does not exclude the possibility of latent infection.

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