Cytopathologic evaluation for the detection of malignant cells in nipple discharge specimens.
Supplies for Submission
No. 2 pencil
Specimen preparation method #1 – Preferred method
Label slides with patient’s full name, first and last, using a pencil.
Express the discharge from the nipple directly onto a properly labeled slide.
Invert a second slide directly on top of the first slide containing the specimen.
Gently pull the two slides apart in opposite directions and immediately spray-fix or place in a container of 95% ethanol.
DO NOT prepare air dried slides. Any slides made should be immediately fixed.
Prepare any additional slides by repeating steps 1-4.
Specimen preparation method #2 – Alternative method
Label all specimen containers with two identifiers. Acceptable identifiers include patient’s name, date of birth, social security number, requisition number, and medical record number.
Collect the nipple discharge directly into a specimen container.
Add CytoLyt® to the specimen, making sure to immerse the specimen completely in CytoLyt®. (For instance, if the collected specimen adheres to the side of the container, wash or rinse the specimen with CytoLyt® for satisfactory preparation.)
Tighten the container cap and gently mix the contents to ensure the discharge is fixed.
Store specimens at room temperature (15 to 30°C) until transported. DO NOT freeze.